Nanoscale 11 (30), 1414114146. If D > 2RF, the curvature is high and mushroom of PEG on the surface of the nanoparticles will form. 
Release 141 (1), 6269. doi:10.1385/0-89603-178-0:81, Filion, M. C., and Phillips, N. C. (1997). Rev. Curr. /Contents [19 0 R 20 0 R 21 0 R] 3, 62976309. J. Range and magnitude of the steric pressure between bilayers containing phospholipids with covalently attached poly(ethylene glycol). doi:10.1002/bit.22858, Meng, Q., Qing, L., Shan, H., Wang, L., Fu, Y., Zhang, Z., et al. 40 (5), 23302344. ACS Appl. doi:10.1038/nm1191, Hu, B., Weng, Y., Xia, X., Liang, X., and Huang, Y. The conjugation of lipid to siRNA could provide a promising way for its therapeutic applications (Kubo et al., 2013). /F11 55 0 R Injection of PEGylated liposomes in rats elicits PEG-specific IgM, which is responsible for rapid elimination of a second dose of PEGylated liposomes. J. Lipid Sci. 9, 13191323. 1380, 211224. 94 (3), 9092. Release 244 (28), 184193. Nanoscale 10 (9), 42584266. endobj Peptides, such as RVG, a 29-amino-acid peptide which was derived from rabies virus glycoproteins, could be immobilized on the surface of siRNA loaded liposome and function as the target ligand to the brain. Cancer Lett. /Font << Sci. doi:10.1021/acs.nanolett.8b01101. stream
Gene-silencing potency of symmetric and asymmetric lipid-conjugated siRNAs and its correlation with dicer recognition. Technol. (2019). /Type /Page Methods for increasing efficacy of lipid formulated siRNA. Bio. Genet. 
endobj /Type /Page (2016). Targeted delivery of packaged siRNA promotes osteogenesis. Other strategies were also used to improve the targeting efficiency. Expet Opin. (2008). On the role of helper lipids in lipid nanoparticle formulations of siRNA. Mater. Leading RNA interference therapeutics part 1: silencing hereditary transthyretin amyloidosis, with a focus on patisiran. (2004). The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Rev. Release 117 (3), 430437. Nanobiomaterials Drug Delivery 9, 75109. During this process, the endosome membrane is destabilized and the structure of the nanoparticle is dissociated for siRNA releasing. lipoplex transfection endocytosis mediated cationic lipids liposomes micellar nonviral molecular Drug Deliv. 
Particle size distribution and surface characteristics are also factors to affect the nature of lipid-based nanoparticles for their distribution (Abra and Hunt, 1981; Rozenberg et al., 1982). 29 (11), 36493657. /Type /Page /ExtGState << colitis nanoparticles assisted cationic tacrolimus ulcerative lipid therapy delivery rsc Nat. (2020). They can self-assemble or encapsulate oligonucleotides through nucleobase interaction. The PEG modified liposome and nucleic acid complex can be formed by electrostatic interaction of anionic nucleic acids with cationic liposomes by simply mixing cationic lipid bilayers and siRNA at expected ratios as shown in Figure 3A (Zhang et al., 2005; Huang, 2009).
doi:10.1021/bm301066w, Nguyen, T., Menocal, E. M., Harborth, J., and Fruehauf, J. H. (2008). J. Pharm. Funct. (2018).
(2010). >> The stability of endosome membrane is thus interrupted and the inverted hexagonal phase (HII) is formed by the cationic lipids from nano vesicles and ionic lipids from endosome membrane. 18 (6), 38143822. 1103 (1), 94100. Colloids Surf. Received: 24 August 2020; Accepted: 09 December 2020;Published: 20 January 2021. doi:10.1038/NMAT3765, Kevin, T., Mahon, K. P., Levins, C. G., Whitehead, K. A., Querbes, W., Dorkin, J. R., et al. Toxicity and immunomodulatory activity of liposomal vectors formulated with cationic lipids toward immune effector cells. TABLE 1. Lett. This formulation process is shown in Figure 3B; siRNA and cationic polymer/or peptide were mixed and condensed to nanoparticles. Nano vectors with the size of less than 6nm are likely to be eliminated after intravenous administration. (2005). Also, the extra positive charges of the surface of the lipoplex could be shielded by PEG, rather than neutralized, is still a concern for systematic delivery of these nucleic acids (Sakurai et al., 2007; Muoz-beda et al., 2012; Kumar et al., 2015). 4 0 obj endobj 40, 11781199. One advantage of this method is that the reactions were typically completed within 3days without further purification for the following experiment. Aptamer-functionalized nanoparticles for drug delivery. endobj Acta Pharm. Rev. Small interfering RNA (siRNA), which is consisted of 2123 nucleotides, has emerged as a powerful tool either in fundamental research or potential medicines for clinical uses in recent decades (Wittrup and Lieberman, 2015). 10 (14), 12331261. &c( hq%24{QGvCRtH$J>27B$9Hf52A;gAU#P%PtJI|H 8 Mater. The principle of LCP nanoparticles delivery system for siRNA is that this vesicle could respond to endosome pH quickly by rapidly dissolving in acidic pH of the calcium phosphate (CaP) (Li et al., 2010; Li et al., 2012). reaction todd atherton scope mechanism applications bactericidal action indicate exhibiting cationic arrows lipids scheme 10 (1), 3747. doi:10.1016/j.jconrel.2006.04.014, Marsh, D., Bartucci, R., and Sportelli, L. (2003). doi:EP0656883A4, Gilbile, D., Docto, D., Kingi, D., Kurniawan, J., Monahan, D., Tang, A., et al. J. Eng. Particles with diameter around 150300nm are easy to distribute in the liver and spleen; other larger molecules are prone to be taken in by monomolecular phagocytic system. (2007). Mol.
Nat.
/Type /Pages Ocular delivery of nucleic acids: antisense oligonucleotides, aptamers and siRNA. A., Rezvaia, S. P., and Khanson, K. P. (1982). 1615 (12), 3359. cationic lipids transfection trimethylammonium dioleoyl viability In the last decade, more efforts have been put to the discovery of the mechanisms, delivery pathways, biological barriers and solutions, and methods for evaluating the behavior and performance of the lipid-based nanoparticles. Application of an HIV gp41-derived peptide for enhanced intracellular trafficking of synthetic gene and siRNA delivery vehicles. 42 (6), 463478. >> (2015). (2005). doi:10.1038/sj.gt.3300947, Lonez, C., Vandenbranden, M., and Ruysschaert, J. M. (2008). doi:10.1093/nar/gkr1002, Ramezanpour, M., Schmidt, M. L., Bodnariuc, I., Kulkarni, J. J. Biomed. Med. /MediaBox [0 0 595 842] The stable nucleic acid lipid particles (SNALPs) were first developed in 2001 (Semple et al., 2001). /Type /Page 1 0 obj
The active RISC containing antisense strand RNA cleavages the target mRNA to induce the silencing effect with several rounds of this action (Dominska and Dykxhoorn, 2010). 21, e3097. Nat. According to the animal experiment, the lipid-based nanoparticle could deliver siRNA to the chronic inflammation sites and provide new method for treatment of arthritis (Aldayel et al., 2018). /F12 56 0 R Nano Lett. 50 (1), 259293. phosphate transfection lipid cationic mrna complexed plasmid enhanced potassium cytotoxicity inhibits 12 0 obj Cell Biol. endobj Au total il y a 59 utilisateurs en ligne :: 2 enregistrs, 0 invisible et 57 invits (daprs le nombre dutilisateurs actifs ces 3 dernires minutes)Le record du nombre dutilisateurs en ligne est de 850, le 05 Avr 2016 20:55 Utilisateurs enregistrs: Google [Bot], kiki37 endobj doi:10.1038/nbt.2634. /Creator (K2 Typesetting Software \(Science Typographers Inc\)) doi:10.1016/j.nano.2016.08.005, Farhood, H., Serbina, N., and Huang, L. (1995). Aptamers are a type of synthetic oligonucleotides with three dimensional folding structures.
(2005). /Contents 24 0 R /Im2 59 0 R (2010). (2014).
(2008). 25 (27), 43524363. While nanoparticles without PEG modification, the radius increased 3nm. -Tocopherol derived lipid dimers as efficient gene transfection agents. 13 (1), 507530. (2011). doi:10.1038/nbt936, Rozenberg, O. Mol. Lipid Res. Cationic lipids may induce toxicity, and the use of these lipids can increase the zeta potential of formed lipid-based nanoparticles, which results in the nonspecific adsorption of components in serum and uptake by RES. doi:10.1021/bc700448h, Landen, C. N., Chavez-Reyes, A., Bucana, C., Schmandt, R., Deavers, M. T., Lopez-Berestein, G., et al. doi:10.1038/ncb1038, Soenen, S. J., Brisson, A. R., and De Cuyper, M. (2009). One example is the design of lipopolyplexes with matrix metalloproteinase (MMP)-cleavable PEG (PPD/PEG5k-MEND), which could show higher tumor accumulation and gene silencing efficiency. doi:10.1016/S0006-3495(95)80369-3, Khatri, N., Baradia, D., Vhora, I., Rathi, M., and Misra, A. J. Pharm. 9 0 obj doi:10.1016/j.addr.2009.04.005, Ewe, A., and Aigner, A. (2019). It is important to learn more about the relationship between structureactivity of the lipids to balance the silencing efficiency and biocompatibility and to know more information for potential toxicity and immunity induced by these materials. Orphanet J. Microbiol. Chem. FIGURE 3. Nanoparticle delivery of HIF1 siRNA combined with photodynamic therapy as a potential treatment strategy for head-and-neck cancer. doi:10.1039/c4ob02063d, Kumar, V., Qin, J., Jiang, Y., Duncan, R. G., Brigham, B., Fishman, S., et al. This method provided an ideal strategy to expand the diversity of lipids for nanoparticle formulation to achieve the high delivery efficiency of siRNA (Kevin et al., 2010; Wang and Huang, 2013a; Uemura et al., 2019; Li et al., 2020; Meel et al., 2020). %PDF-1.7 doi:10.1007/978-1-4939-3197-2_18, Wittrup, A., and Lieberman, J. In this work, we summarize the mechanism and delivery pathway of siRNA, barriers of siRNA target delivery, rationale design of lipid-based nanoparticles, and challenge and solution in siRNA delivery, which were associated with PEGylation. /Resources 25 0 R Cl1/fE3 1;yY.~ imEs>P@"rxYw~;EM.VMii5Pg(y6mM doi:10.1016/S0005-2736(03)00197-4, Mcnamara, J. O., Andrechek, E. R., Wang, Y., Viles, K. D., Rempel, R. E., Gilboa, E., et al. FIGURE 1. 61 (9), 721731. siRNA delivery and variety of lipid-based nanoparticles are developed in recent years. Annu. Mol. Sci. Drug Deliv. << Implications of pharmacokinetic behavior of lipoplex for its inflammatory toxicity. /Kids [6 0 R 7 0 R 8 0 R 9 0 R 10 0 R 11 0 R 12 0 R 13 0 R 14 0 R 15 0 R] Release 277, 173182. doi:10.1016/j.colsurfb.2018.07.008, Yang, S., Chen, Y., Ahmadie, R., and Ho, E. A. (2007). Although some siRNAs are injected locally, such as age-related macular degeneration (AMD) treatment, most of these therapeutic agents need to be administrated systematically to circulate and reach the target cells (Fattal and Bochot, 2006; Nguyen et al., 2008; Whitehead et al., 2009; Yang et al., 2013). N. Engl. >> cationic dotap lipid representation hydrophobic 121 (6), 24012412. doi:10.1021/acs.langmuir.9b02163, Hamby, K. (1995). Due to the nature of these bio-molecules and obstacles existing in the delivery pathways, efficient delivery system is needed to help these therapeutic agents to reach the target sites safely and accurately (Raye et al., 2018). By using double click for the modification, these nanoparticles can achieve desired bio-distribution and intracellular delivery of siRNA (Klein et al., 2018). rsc abstract mpeg cationic lipid paac release (2013). U.S.A. 75, 285288. It could recognize tumor cells to facilitate cell attachment of lipid-based nanoparticle with high efficiency (Mendon et al., 2010). Nature 452 (7187), 591597. J. Med. The curvature is high and mushroom of PEG on the surface of the nanoparticles will form when the value of the D > 2RF. doi:10.1021/bc0603539, Dominska, M., and Dykxhoorn, D. M. (2010). doi:10.1038/nbt.1602, Semple, S. C., Klimuk, S. K., Harasym, T. O., Dos Santos, N., Ansell, S. M., Wong, K. F., et al. U. S. Patent No 20190153443. Activation of the interferon system by short-interfering RNAs. /Type /Page /F13 57 0 R (2010). /Count 2 Brain-targeting gene delivery using a rabies virus glycoprotein peptide modulated hollow liposome: bio-behavioral study. endobj
Mouse model with collagen-induced arthritis was used to investigate the efficiency of this siRNA loaded nanoparticle. Folate receptor-directed orthogonal click-functionalization of siRNA lipopolyplexes for tumor cell killing in vivo. /Parent 4 0 R
doi:10.1002/adfm.201601703, Tao, Y., Han, J., and Dou, H. (2012). /Title (PII: S0005-2736\(97\)00126-0) Biotechnol. Overcoming the inflammatory toxicity in cationic lipid vector-mediated systemic gene delivery. Induction of an interferon response by RNAi vectors in mammalian cells. cationic lipid dope gene lipids nanoparticles Parlez-en ! No use, distribution or reproduction is permitted which does not comply with these terms. 47 (5), 340347. Biochim. Cell. Acta. Oncomedicine 3, 4858. doi:10.1021/acsnano.5b01326, Perrie, Y., Frederik, P. M., and Gregoriadis, G. (2001). >> doi:10.1172/JCI45876, Whitehead, K. A., Langer, R., and Anderson, D. G. (2009). (2004). Drug Discov. For disease cell reorganization, the targeting molecules were selected and modified on the surface of lipid-based nanoparticles to enhance the attachment of the targeting cells. Development of a novel systemic gene delivery system for cancer therapy with a tumor-specific cleavable PEG-lipid. 23 (8), 946947. J. Hoboken, NJ: John Wiley and Sons. <> The structure of (A) lipoplex and SNALP, (B) LPD, and (C) LCP, and formulation process of (B) lipopolyplex and (C) LCP (Xia et al., 2015) (Kumar et al., 2015). J. Gene Med. In previous work, the PEGylation on the surface of liposome for siRNA was well introduced to illustrate the effects in different kinds of liposomes (Yuen et al., 2013; Xia et al., 2015). (2018). doi:10.1211/0022357021778817, Davis, M. E. (2007). >> Koh, C. G., Zhang, X., Liu, S., Golan, S., Yu, B., Yang, X., et al. It is reported that mono-disperse precise carriers assemble by using the forming materials which conjugated two oleic acids to cationizable oligominnoamide, and the targeting anchor atherosclerotic plaque-specific peptides can couple to the end of the synthesized T-sharp configuration lipo-oligomers to recognize the targeting cell. doi:10.1039/c9nr09347h, Kumar, K., Maiti, B., Kondaiah, P., and Bhattacharya, S. (2015). doi:10.1038/nrm1646, Aldayel, A. M., OMary, H. L., Valdes, S. A., Xu, L., Thakkar, S. G., Mustafa, B. E., et al. J. Contr.
endobj 8xV(=%kl^X=sq5Ys yYP~dyzs 'wGG ?-|JYEM(f!bt2*Z)v|wRKMW+'>,eg5.
Research works on the formation and morphology of LNPs during formulation process were investigated. Lipid nanoparticles for the delivery of nucleic acids. 285 (29), 2263922650. J. Contr. ONPATTRO (patisiran) has been approved by the United States Food and Drug Administration (FDA) as the first RNAi-based drug to be used clinically in 2018. WY conducted the review and XG wrote the draft while BZ and LC revised the manuscript and helped with the figures. endobj doi:10.1016/j.biomaterials.2018.03.031, Kleinman, M. E., Yamada, K., Takeda, A., Chandrasekaran, V., Nozaki, M., Baffi, J. Construction of a targeting nanoparticle of 3, 3-Bis-Peptide-siRNA conjugate/mixed lipid with postinserted DSPE-PEG2000-cRGD. Release. Formation of ion-pair between cationic lipid from lipoplex and membrane lipid. Drug Deliv. cationic lipids dotma
Biochim. Enhanced delivery of siRNA to triple negative breast cancer cells in vitro and in vivo through functionalizing lipid-coated calcium phosphate nanoparticles with dual target ligands. >> ACS Appl.
Patisiran was applied for treatment of TTR mediated amyloidosis which could reduce the expression of target protein mutated transthyretin (TTR) to 80% at the dose of 0.3mg/kg every 3weeks (Ole et al., 2015). Biomol. Interfaces 6 (24), 2183221841. << The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer. (2015). doi:10.1038/nrg3978, Wolfrum, C., Shi, S., Jayaprakash, K. N., Jayaraman, M., Wang, G., Pandey, R. K., et al. RNA therapeutics: beyond RNA interference and antisense oligonucleotides. Pharmaceut. Secondly, these nanoparticles should be easy to escape from the endosome (or by other biological pathways) and to release enough siRNA into the cytoplasm. To realize the targeting delivery of siRNA to diseased cells, different types of molecules such as chemical molecules, peptides, antibodies, and aptamers were immobilized on the surface of the lipid-based nanoparticle as anchors to help these nano vesicles to find the targeting cells. Repeated injections of PEG-PE liposomes generate anti-PEG antibodies. Most of the cationic lipid-based nanoparticles are prone to evoke toxicity because of using cationic lipids and easy to aggregate with serum components due to extra positive surface charges (Gebeyehu et al., 1994; Scheule et al., 1997; Lv et al., 2006). 11 (4), 196. doi:10.1038/nrrheum.2015.19, Endoh, T., and Ohtsuki, T. (2009). 5 (9), 834839. Biophys. (2014). Effect of surface properties on liposomal siRNA delivery. Huang, L. (2009). 8 (2), 129138. Cell Sci. These nanoparticles are easy to be scaled up and used. doi:10.1016/B978-0-323-42866-8.00003-4, Petrova, N. S., Chernikov, I. V., Meschaninova, M. I., Dovydenko, I. S., Venyaminova, A. G., Zenkova, M. A., et al. /Pages 2 0 R The targeting and antitumor efficiency was increased due to the slight negative zeta potentials and uniform sizes of the nanoparticles. Rev. B.
To address this issue, PEGylation was widely used to increase the circulation time and efficiency in vivo. Toxicol. doi:10.1016/0005-2760(81)90311-8, Abreu-Goodger, C., van Dongen, S., and Enright, A. J. Dev. Low incorporation of PEG could not fully protect the nanoparticles to interact with serum proteins because PEG is unable to provide ideal steric stabilization for nanoparticles. However, the increased proportion of PEG on the surface of the lipid-based nanoparticles may reduce the mechanical stability and induce the dissociation.
doi:10.1016/j.ijpharm.2010.03.047, Klein, P. M., Kern, S., Lee, D. J., Schmaus, J., Hhn, M., Gorges, J., et al. 359 (1), 6574. Chem. PEGylation of the lipid can shield the positive charges with increased steric stability. /F5 49 0 R How to overcome obstacles and barriers for efficient target delivery of siRNA remains unsolved (Whitehead et al., 2011; Kanasty et al., 2013; Khvorova et al., 2014; Duarte Joao, 2015).
Release 141 (1), 6269. doi:10.1385/0-89603-178-0:81, Filion, M. C., and Phillips, N. C. (1997). Rev. Curr. /Contents [19 0 R 20 0 R 21 0 R] 3, 62976309. J. Range and magnitude of the steric pressure between bilayers containing phospholipids with covalently attached poly(ethylene glycol). doi:10.1002/bit.22858, Meng, Q., Qing, L., Shan, H., Wang, L., Fu, Y., Zhang, Z., et al. 40 (5), 23302344. ACS Appl. doi:10.1038/nm1191, Hu, B., Weng, Y., Xia, X., Liang, X., and Huang, Y. The conjugation of lipid to siRNA could provide a promising way for its therapeutic applications (Kubo et al., 2013). /F11 55 0 R Injection of PEGylated liposomes in rats elicits PEG-specific IgM, which is responsible for rapid elimination of a second dose of PEGylated liposomes. J. Lipid Sci. 9, 13191323. 1380, 211224. 94 (3), 9092. Release 244 (28), 184193. Nanoscale 10 (9), 42584266. endobj Peptides, such as RVG, a 29-amino-acid peptide which was derived from rabies virus glycoproteins, could be immobilized on the surface of siRNA loaded liposome and function as the target ligand to the brain. Cancer Lett. /Font << Sci. doi:10.1021/acs.nanolett.8b01101. stream
Gene-silencing potency of symmetric and asymmetric lipid-conjugated siRNAs and its correlation with dicer recognition. Technol. (2019). /Type /Page Methods for increasing efficacy of lipid formulated siRNA. Bio. Genet. endobj /Type /Page (2016). Targeted delivery of packaged siRNA promotes osteogenesis. Other strategies were also used to improve the targeting efficiency. Expet Opin. (2008). On the role of helper lipids in lipid nanoparticle formulations of siRNA. Mater. Leading RNA interference therapeutics part 1: silencing hereditary transthyretin amyloidosis, with a focus on patisiran. (2004). The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Rev. Release 117 (3), 430437. Nanobiomaterials Drug Delivery 9, 75109. During this process, the endosome membrane is destabilized and the structure of the nanoparticle is dissociated for siRNA releasing. lipoplex transfection endocytosis mediated cationic lipids liposomes micellar nonviral molecular Drug Deliv. 
Particle size distribution and surface characteristics are also factors to affect the nature of lipid-based nanoparticles for their distribution (Abra and Hunt, 1981; Rozenberg et al., 1982). 29 (11), 36493657. /Type /Page /ExtGState << colitis nanoparticles assisted cationic tacrolimus ulcerative lipid therapy delivery rsc Nat. (2020). They can self-assemble or encapsulate oligonucleotides through nucleobase interaction. The PEG modified liposome and nucleic acid complex can be formed by electrostatic interaction of anionic nucleic acids with cationic liposomes by simply mixing cationic lipid bilayers and siRNA at expected ratios as shown in Figure 3A (Zhang et al., 2005; Huang, 2009). doi:10.1021/bm301066w, Nguyen, T., Menocal, E. M., Harborth, J., and Fruehauf, J. H. (2008). J. Pharm. Funct. (2018).
(2010). >> The stability of endosome membrane is thus interrupted and the inverted hexagonal phase (HII) is formed by the cationic lipids from nano vesicles and ionic lipids from endosome membrane. 18 (6), 38143822. 1103 (1), 94100. Colloids Surf. Received: 24 August 2020; Accepted: 09 December 2020;Published: 20 January 2021. doi:10.1038/NMAT3765, Kevin, T., Mahon, K. P., Levins, C. G., Whitehead, K. A., Querbes, W., Dorkin, J. R., et al. Toxicity and immunomodulatory activity of liposomal vectors formulated with cationic lipids toward immune effector cells. TABLE 1. Lett. This formulation process is shown in Figure 3B; siRNA and cationic polymer/or peptide were mixed and condensed to nanoparticles. Nano vectors with the size of less than 6nm are likely to be eliminated after intravenous administration. (2005). Also, the extra positive charges of the surface of the lipoplex could be shielded by PEG, rather than neutralized, is still a concern for systematic delivery of these nucleic acids (Sakurai et al., 2007; Muoz-beda et al., 2012; Kumar et al., 2015). 4 0 obj endobj 40, 11781199. One advantage of this method is that the reactions were typically completed within 3days without further purification for the following experiment. Aptamer-functionalized nanoparticles for drug delivery. endobj Acta Pharm. Rev. Small interfering RNA (siRNA), which is consisted of 2123 nucleotides, has emerged as a powerful tool either in fundamental research or potential medicines for clinical uses in recent decades (Wittrup and Lieberman, 2015). 10 (14), 12331261. &c( hq%24{QGvCRtH$J>27B$9Hf52A;gAU#P%PtJI|H 8 Mater. The principle of LCP nanoparticles delivery system for siRNA is that this vesicle could respond to endosome pH quickly by rapidly dissolving in acidic pH of the calcium phosphate (CaP) (Li et al., 2010; Li et al., 2012). reaction todd atherton scope mechanism applications bactericidal action indicate exhibiting cationic arrows lipids scheme 10 (1), 3747. doi:10.1016/j.jconrel.2006.04.014, Marsh, D., Bartucci, R., and Sportelli, L. (2003). doi:EP0656883A4, Gilbile, D., Docto, D., Kingi, D., Kurniawan, J., Monahan, D., Tang, A., et al. J. Eng. Particles with diameter around 150300nm are easy to distribute in the liver and spleen; other larger molecules are prone to be taken in by monomolecular phagocytic system. (2007). Mol.
Nat.
/Type /Pages Ocular delivery of nucleic acids: antisense oligonucleotides, aptamers and siRNA. A., Rezvaia, S. P., and Khanson, K. P. (1982). 1615 (12), 3359. cationic lipids transfection trimethylammonium dioleoyl viability In the last decade, more efforts have been put to the discovery of the mechanisms, delivery pathways, biological barriers and solutions, and methods for evaluating the behavior and performance of the lipid-based nanoparticles. Application of an HIV gp41-derived peptide for enhanced intracellular trafficking of synthetic gene and siRNA delivery vehicles. 42 (6), 463478. >> (2015). (2005). doi:10.1038/sj.gt.3300947, Lonez, C., Vandenbranden, M., and Ruysschaert, J. M. (2008). doi:10.1093/nar/gkr1002, Ramezanpour, M., Schmidt, M. L., Bodnariuc, I., Kulkarni, J. J. Biomed. Med. /MediaBox [0 0 595 842] The stable nucleic acid lipid particles (SNALPs) were first developed in 2001 (Semple et al., 2001). /Type /Page 1 0 obj
The active RISC containing antisense strand RNA cleavages the target mRNA to induce the silencing effect with several rounds of this action (Dominska and Dykxhoorn, 2010). 21, e3097. Nat. According to the animal experiment, the lipid-based nanoparticle could deliver siRNA to the chronic inflammation sites and provide new method for treatment of arthritis (Aldayel et al., 2018). /F12 56 0 R Nano Lett. 50 (1), 259293. phosphate transfection lipid cationic mrna complexed plasmid enhanced potassium cytotoxicity inhibits 12 0 obj Cell Biol. endobj Au total il y a 59 utilisateurs en ligne :: 2 enregistrs, 0 invisible et 57 invits (daprs le nombre dutilisateurs actifs ces 3 dernires minutes)Le record du nombre dutilisateurs en ligne est de 850, le 05 Avr 2016 20:55 Utilisateurs enregistrs: Google [Bot], kiki37 endobj doi:10.1038/nbt.2634. /Creator (K2 Typesetting Software \(Science Typographers Inc\)) doi:10.1016/j.nano.2016.08.005, Farhood, H., Serbina, N., and Huang, L. (1995). Aptamers are a type of synthetic oligonucleotides with three dimensional folding structures. 
(2005). /Contents 24 0 R /Im2 59 0 R (2010). (2014).
(2008). 25 (27), 43524363. While nanoparticles without PEG modification, the radius increased 3nm. -Tocopherol derived lipid dimers as efficient gene transfection agents. 13 (1), 507530. (2011). doi:10.1038/nbt936, Rozenberg, O. Mol. Lipid Res. Cationic lipids may induce toxicity, and the use of these lipids can increase the zeta potential of formed lipid-based nanoparticles, which results in the nonspecific adsorption of components in serum and uptake by RES. doi:10.1021/bc700448h, Landen, C. N., Chavez-Reyes, A., Bucana, C., Schmandt, R., Deavers, M. T., Lopez-Berestein, G., et al. doi:10.1038/ncb1038, Soenen, S. J., Brisson, A. R., and De Cuyper, M. (2009). One example is the design of lipopolyplexes with matrix metalloproteinase (MMP)-cleavable PEG (PPD/PEG5k-MEND), which could show higher tumor accumulation and gene silencing efficiency. doi:10.1016/S0006-3495(95)80369-3, Khatri, N., Baradia, D., Vhora, I., Rathi, M., and Misra, A. J. Pharm. 9 0 obj doi:10.1016/j.addr.2009.04.005, Ewe, A., and Aigner, A. (2019). It is important to learn more about the relationship between structureactivity of the lipids to balance the silencing efficiency and biocompatibility and to know more information for potential toxicity and immunity induced by these materials. Orphanet J. Microbiol. Chem. FIGURE 3. Nanoparticle delivery of HIF1 siRNA combined with photodynamic therapy as a potential treatment strategy for head-and-neck cancer. doi:10.1039/c4ob02063d, Kumar, V., Qin, J., Jiang, Y., Duncan, R. G., Brigham, B., Fishman, S., et al. This method provided an ideal strategy to expand the diversity of lipids for nanoparticle formulation to achieve the high delivery efficiency of siRNA (Kevin et al., 2010; Wang and Huang, 2013a; Uemura et al., 2019; Li et al., 2020; Meel et al., 2020). %PDF-1.7 doi:10.1007/978-1-4939-3197-2_18, Wittrup, A., and Lieberman, J. In this work, we summarize the mechanism and delivery pathway of siRNA, barriers of siRNA target delivery, rationale design of lipid-based nanoparticles, and challenge and solution in siRNA delivery, which were associated with PEGylation. /Resources 25 0 R Cl1/fE3 1;yY.~ imEs>P@"rxYw~;EM.VMii5Pg(y6mM doi:10.1016/S0005-2736(03)00197-4, Mcnamara, J. O., Andrechek, E. R., Wang, Y., Viles, K. D., Rempel, R. E., Gilboa, E., et al. FIGURE 1. 61 (9), 721731. siRNA delivery and variety of lipid-based nanoparticles are developed in recent years. Annu. Mol. Sci. Drug Deliv. << Implications of pharmacokinetic behavior of lipoplex for its inflammatory toxicity. /Kids [6 0 R 7 0 R 8 0 R 9 0 R 10 0 R 11 0 R 12 0 R 13 0 R 14 0 R 15 0 R] Release 277, 173182. doi:10.1016/j.colsurfb.2018.07.008, Yang, S., Chen, Y., Ahmadie, R., and Ho, E. A. (2007). Although some siRNAs are injected locally, such as age-related macular degeneration (AMD) treatment, most of these therapeutic agents need to be administrated systematically to circulate and reach the target cells (Fattal and Bochot, 2006; Nguyen et al., 2008; Whitehead et al., 2009; Yang et al., 2013). N. Engl. >> cationic dotap lipid representation hydrophobic 121 (6), 24012412. doi:10.1021/acs.langmuir.9b02163, Hamby, K. (1995). Due to the nature of these bio-molecules and obstacles existing in the delivery pathways, efficient delivery system is needed to help these therapeutic agents to reach the target sites safely and accurately (Raye et al., 2018). By using double click for the modification, these nanoparticles can achieve desired bio-distribution and intracellular delivery of siRNA (Klein et al., 2018). rsc abstract mpeg cationic lipid paac release (2013). U.S.A. 75, 285288. It could recognize tumor cells to facilitate cell attachment of lipid-based nanoparticle with high efficiency (Mendon et al., 2010). Nature 452 (7187), 591597. J. Med. The curvature is high and mushroom of PEG on the surface of the nanoparticles will form when the value of the D > 2RF. doi:10.1021/bc0603539, Dominska, M., and Dykxhoorn, D. M. (2010). doi:10.1038/nbt.1602, Semple, S. C., Klimuk, S. K., Harasym, T. O., Dos Santos, N., Ansell, S. M., Wong, K. F., et al. U. S. Patent No 20190153443. Activation of the interferon system by short-interfering RNAs. /Type /Page /F13 57 0 R (2010). /Count 2 Brain-targeting gene delivery using a rabies virus glycoprotein peptide modulated hollow liposome: bio-behavioral study. endobj
Mouse model with collagen-induced arthritis was used to investigate the efficiency of this siRNA loaded nanoparticle. Folate receptor-directed orthogonal click-functionalization of siRNA lipopolyplexes for tumor cell killing in vivo. /Parent 4 0 R
doi:10.1002/adfm.201601703, Tao, Y., Han, J., and Dou, H. (2012). /Title (PII: S0005-2736\(97\)00126-0) Biotechnol. Overcoming the inflammatory toxicity in cationic lipid vector-mediated systemic gene delivery. Induction of an interferon response by RNAi vectors in mammalian cells. cationic lipid dope gene lipids nanoparticles Parlez-en ! No use, distribution or reproduction is permitted which does not comply with these terms. 47 (5), 340347. Biochim. Cell. Acta. Oncomedicine 3, 4858. doi:10.1021/acsnano.5b01326, Perrie, Y., Frederik, P. M., and Gregoriadis, G. (2001). >> doi:10.1172/JCI45876, Whitehead, K. A., Langer, R., and Anderson, D. G. (2009). (2004). Drug Discov. For disease cell reorganization, the targeting molecules were selected and modified on the surface of lipid-based nanoparticles to enhance the attachment of the targeting cells. Development of a novel systemic gene delivery system for cancer therapy with a tumor-specific cleavable PEG-lipid. 23 (8), 946947. J. Hoboken, NJ: John Wiley and Sons. <> The structure of (A) lipoplex and SNALP, (B) LPD, and (C) LCP, and formulation process of (B) lipopolyplex and (C) LCP (Xia et al., 2015) (Kumar et al., 2015). J. Gene Med. In previous work, the PEGylation on the surface of liposome for siRNA was well introduced to illustrate the effects in different kinds of liposomes (Yuen et al., 2013; Xia et al., 2015). (2018). doi:10.1211/0022357021778817, Davis, M. E. (2007). >> Koh, C. G., Zhang, X., Liu, S., Golan, S., Yu, B., Yang, X., et al. It is reported that mono-disperse precise carriers assemble by using the forming materials which conjugated two oleic acids to cationizable oligominnoamide, and the targeting anchor atherosclerotic plaque-specific peptides can couple to the end of the synthesized T-sharp configuration lipo-oligomers to recognize the targeting cell. doi:10.1039/c9nr09347h, Kumar, K., Maiti, B., Kondaiah, P., and Bhattacharya, S. (2015). doi:10.1038/nrm1646, Aldayel, A. M., OMary, H. L., Valdes, S. A., Xu, L., Thakkar, S. G., Mustafa, B. E., et al. J. Contr.
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Research works on the formation and morphology of LNPs during formulation process were investigated. Lipid nanoparticles for the delivery of nucleic acids. 285 (29), 2263922650. J. Contr. ONPATTRO (patisiran) has been approved by the United States Food and Drug Administration (FDA) as the first RNAi-based drug to be used clinically in 2018. WY conducted the review and XG wrote the draft while BZ and LC revised the manuscript and helped with the figures. endobj doi:10.1016/j.biomaterials.2018.03.031, Kleinman, M. E., Yamada, K., Takeda, A., Chandrasekaran, V., Nozaki, M., Baffi, J. Construction of a targeting nanoparticle of 3, 3-Bis-Peptide-siRNA conjugate/mixed lipid with postinserted DSPE-PEG2000-cRGD. Release. Formation of ion-pair between cationic lipid from lipoplex and membrane lipid. Drug Deliv. cationic lipids dotma
Biochim. Enhanced delivery of siRNA to triple negative breast cancer cells in vitro and in vivo through functionalizing lipid-coated calcium phosphate nanoparticles with dual target ligands. >> ACS Appl.
Patisiran was applied for treatment of TTR mediated amyloidosis which could reduce the expression of target protein mutated transthyretin (TTR) to 80% at the dose of 0.3mg/kg every 3weeks (Ole et al., 2015). Biomol. Interfaces 6 (24), 2183221841. << The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer. (2015). doi:10.1038/nrg3978, Wolfrum, C., Shi, S., Jayaprakash, K. N., Jayaraman, M., Wang, G., Pandey, R. K., et al. RNA therapeutics: beyond RNA interference and antisense oligonucleotides. Pharmaceut. Secondly, these nanoparticles should be easy to escape from the endosome (or by other biological pathways) and to release enough siRNA into the cytoplasm. To realize the targeting delivery of siRNA to diseased cells, different types of molecules such as chemical molecules, peptides, antibodies, and aptamers were immobilized on the surface of the lipid-based nanoparticle as anchors to help these nano vesicles to find the targeting cells. Repeated injections of PEG-PE liposomes generate anti-PEG antibodies. Most of the cationic lipid-based nanoparticles are prone to evoke toxicity because of using cationic lipids and easy to aggregate with serum components due to extra positive surface charges (Gebeyehu et al., 1994; Scheule et al., 1997; Lv et al., 2006). 11 (4), 196. doi:10.1038/nrrheum.2015.19, Endoh, T., and Ohtsuki, T. (2009). 5 (9), 834839. Biophys. (2014). Effect of surface properties on liposomal siRNA delivery. Huang, L. (2009). 8 (2), 129138. Cell Sci. These nanoparticles are easy to be scaled up and used. doi:10.1016/B978-0-323-42866-8.00003-4, Petrova, N. S., Chernikov, I. V., Meschaninova, M. I., Dovydenko, I. S., Venyaminova, A. G., Zenkova, M. A., et al. /Pages 2 0 R The targeting and antitumor efficiency was increased due to the slight negative zeta potentials and uniform sizes of the nanoparticles. Rev. B.
To address this issue, PEGylation was widely used to increase the circulation time and efficiency in vivo. Toxicol. doi:10.1016/0005-2760(81)90311-8, Abreu-Goodger, C., van Dongen, S., and Enright, A. J. Dev. Low incorporation of PEG could not fully protect the nanoparticles to interact with serum proteins because PEG is unable to provide ideal steric stabilization for nanoparticles. However, the increased proportion of PEG on the surface of the lipid-based nanoparticles may reduce the mechanical stability and induce the dissociation.
doi:10.1016/j.ijpharm.2010.03.047, Klein, P. M., Kern, S., Lee, D. J., Schmaus, J., Hhn, M., Gorges, J., et al. 359 (1), 6574. Chem. PEGylation of the lipid can shield the positive charges with increased steric stability. /F5 49 0 R How to overcome obstacles and barriers for efficient target delivery of siRNA remains unsolved (Whitehead et al., 2011; Kanasty et al., 2013; Khvorova et al., 2014; Duarte Joao, 2015).