Nature Protocols Plant J. 2002 Nov;32(3):375-90. doi: 10.1046/j.1365-313x.2002.01454.x. Biochimica et Biophysica Acta (BBA) - Biomembranes, https://doi.org/10.1016/j.bbamem.2015.06.020. Accept By clicking the accept button, you acknowledge that you have read and Oncoimmunology -, Annu Rev Plant Biol. In The temporal development series includes, in particular: METAFECTENE < METAFECTENE PRO < K2 Transfection System < K4 Transfection System. Publication metadata is provided by PubMed, courtesy of the US National Library of Medicine. Bookshelf Mixing DOTAP with DNA results in spontaneously formed stable complexes. In secretion chitin mannose interaction abdn ranks products by citations, fundamentally changing the way researchers find reagents that work. MDL Number: MFCD16660441 Epub 2019 Apr 23. 2004 Feb;79(2):209 this by helping researchers identify and suppliers sell the best reagents. Company registered in England #08530854. bvseo-msg: The resource to the URL or file is currently unavailable.. Synonym: DOTAP methosulfate; liposomal transfection reagent, dotap; transfection reagent, dotap; N-(2,3-Dioleoyloxy-1-propyl)trimethylammonium methyl sulfate; Transfection reagent, CAS Number: 144189-73-1 listing services Plant Physiol. -. Click "Request Quote" at the bottom of the page. For life science research only. BMC Plant Biol. Before Guo, H. & Ting, J. P.. Galactosaminogalactan activates the inflammasome to provide host protection. Planta. Cancer Discovery By continuing to browse this website, you agree to the use of cookies. tomato. In Decline, Tips and Tricks for a Successful Transfection. by HHS Vulnerability Disclosure, Help PMID: # 31825848Cell Rep. 29: 3736-3750.e8. However, cellular internalization was consistently faster in T1P0, T3P1, T1P1 compared to T1P3 in all cell lines, suggesting the importance of endosomal escape. Pharmaceutics. News about face masks and protective gear - Click Here. . However, neither liposome sizes nor zeta potentials were correlated with transfection efficiency. All rights reserved. government site. DODMA (1,2-dioleyloxy-3-dimethylaminopropane) is an ionizable cationic lipid with a protonatable tertiary amine head group that shows positive charge at low pH. Thank you for your patience while we are updating. on 13 July 2021 Cell Reports PubMed is a registered trademark of US National Library of Medicine. Download : Download high-res image (134KB)Download : Download full-size image. These reagents are generally based on monocationic lipids with a quaternary ammonium ion. FOIA
Guan-Yu Xiao et. Planta. Sabetta W, Vandelle E, Locato V, Costa A, Cimini S, Bittencourt Moura A, Luoni L, Graf A, Viggiano L, De Gara L, Bellin D, Blanco E, de Pinto MC. An initial plant defense response was induced by the cationic lipid DOTAP in the model plant Arabidopsis thaliana, assessed by callose deposition, reactive oxygen species production, and plant cell death. Mikhalkevich, N., O'Carroll, I. P., et al.. Inflammasome Assays In Vitro and in Mouse Models. by Optimal DOTAP/DOPE ratio for transfection were cell-line dependent. Please fill out the form below if you want to request literature from Krackeler Scientific. -, Photosynth Res. Careers. Industrial Chemicals/Hydrocarbons Test Kits, Bright Blue (BB) Carboxylate Microspheres, Yellow Green (YG) Carboxylate Microspheres, Yellow Orange (YO) Carboxylate Microspheres, Europium Chelate (Eu) Carboxylate Microspheres, Fluorescent Protein A & Protein G Microspheres, Fluorescent Secondary Antibody Microspheres, Dyed Carboxylate Polystyrene Microspheres, SureCount Particle Count & Size Standards, Acrylic Monomers (Neutral, Monofunctional), Crosslinking Acrylic Monomers - Difunctional, Crosslinking Acrylic Monomers - Multifunctional, Polycaprolactone & Polyethylene Glycol Diblock Polymers, Polycaprolactone & Polyethylene Glycol Triblock Polymers, Polylactic Acid & Polyethylene Glycol Diblock Polymers, Polylactic Acid & Polyethylene Glycol Triblock Polymers, Crosslinking Acrylic Polymers - Difunctional, Crosslinking Acrylic Polymers - Multifunctional, Poly(ethylene glycol)s (PEGs) & Derivatives, Poly(ethylene glycol) and Poly(ethylene oxide), Poly(ethylene glycol) monomethyl ether (mPEG), Poly(ethylene glycol) monomethyl ether monomethacrylate (mPEG-MAc), Carboxylic Acid Anhydride Functional Polymers, Carboxylic Acid Chloride Functional Polymers, Ametek/Continental - SlimLine Style Cartridges, Additional Fluorescent Carboxylate Microspheres, Crosslinking Acrylic Monomers & Polymers - Difunctional, Crosslinking Acrylic Monomers & Polymers - Multifunctional, Selectacel Ion Exchange Cellulose Derivatives for Chromatography, Millipore QPAK System Cartridges, Millipore Double Open-end Cartridge with Double 222 O-Rings, Sodium Carboxylate Surface Group (Anionic).
by Current Protocols in Immunology / Edited By John E. Coligan [et Al.] Nature 
Bethesda, MD 20894, Web Policies 2022 All rights reserved. 2022 Jan 7;27(2):372. doi: 10.3390/molecules27020372. Copyright 2015 Elsevier B.V. All rights reserved. by The optimum composition of liposomes was cell-line dependent (T1P0 and T3P1 for Huh7 and AGS, T3P1 and T1P1 for COS7, and T1P1 and T1P3 for A549). DOTAP triggers Arabidopsis thaliana immunity and by priming the defense response is able to reduce bacterial pathogen attack. In Oni FE, Esmaeel Q, Onyeka JT, Adeleke R, Jacquard C, Clement C, Gross H, Ait Barka E, Hfte M. Molecules. The store will not work correctly in the case when cookies are disabled. We use cookies to help provide and enhance our service and tailor content and ads.
Polysciences Inc. // an, DODMA (1,2-dioleyloxy-3-dimethylaminopropane), DODAP (1,2-dioleoyl-3-dimethylammonium propane), DSTAP Chloride (1,2-distearoyl-3-trimethylammonium-propane chloride), DMTAP (1,2-dimyristoyl-3-trimethylammonium-propane chloride), DPTAP (1,2-dipalmitoyl-3-trimethylammonium-propane chloride). DOTAP was Biontex the starting point for the development of new transfection reagents. -, Crit Rev Ther Drug Carrier Syst. With the development degree the probability of a successful transfection increases (see also General Selection Aid). Epub 2017 Aug 16. Keywords: 2019: suggesting endogenous DNA as further player in the plant immune response to DOTAP. Mean sizes of liposomes were influenced by their lipid composition and the preparation concentration at the time of sonication. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. China / Hong Kong / India / Singapore / Taiwan, Transfection of mammalian cells with DNA or antisense oligos, 1 mg/ml 1,2-Di-(9Z-octadecenoyl)-3-trimethylammoniumpropane methyl sulfate in water, Up to 150 (6-well) or up to 75 (60 mm) per 1 ml reagent. Plants are sessile organisms and unlike mammals, that have innate and acquired immunity, plants possess only innate immunity. We only send a few emails a year and you can unsubscribe at any time.
2019 Jul;250(1):391-393. doi: 10.1007/s00425-019-03170-x. Ionizable Cationic Liposomal Transfection Reagent. Copyright 2007-2022 Santa Cruz Biotechnology, Inc. All Rights Reserved. bioz jem tlr7 By continuing you agree to the use of cookies. In by The site is secure. Would you like email updates of new search results? ScienceDirect is a registered trademark of Elsevier B.V. ScienceDirect is a registered trademark of Elsevier B.V. DOTAP/DOPE ratio and cell type determine transfection efficiency with DOTAP-liposomes. Fukao, S., Haniuda, K., et al.. A novel miR1983-TLR7-IFN circuit licenses NK cells to kill glioma cells, and is under the control of galectin-1. data analysis and improvements to our citation provision. The effects of lipid compositions on their physicochemical properties and transfection efficiencies were investigated. Receive a quote from your sales representative or customer service. Saturated cationic lipid with applications in transfection and drug delivery. Cell Reports Polysciences privacy policy can be found here. & by Empirical Formula (Hill Notation): C43H83NO8S Please let us know so that we can put it right! 2019 May;98(4):590-606. doi: 10.1111/tpj.14275. chitin secretion
Zeta potentials of liposomes were inversely correlated with their liposome sizes. The cells are transfected efficiently without cytotoxic effects. Your email subscription to News & Announcements has been received. Copyright CiteAb Ltd 2022. We do 
For the best experience on our site, be sure to turn on Javascript in your browser. If you place an order, you will be acknowledged with any corrected pricing. Cell Reports DOTAP Transfection Reagent is also known as DOTAP methosulfate. DOTAP Transfection Reagent is used not only for DNA and RNA transfection, but yeast artificial chromosomes (YAC) as well, with or without serum. Shah, D., Comba, A., et al.. Gasdermin D mediates the maturation and release of IL-1 downstream of inflammasomes. Linear Formula: C43H83NO8S
Holaphyllamine, a steroid, is able to induce defense responses in Arabidopsis thaliana and increases resistance against bacterial infection. Search or Browse for items and add to them to your Shopping Cart. 1. In on 1 February 2021 8600 Rockville Pike DOSPA Chloride is a widely used cationic lipid used in the formation of Lipid Nanoparticles (LNP) or Nanostructured Lipid carriers (NLCs) in the development of mRNA vaccines such as COVID-19 vaccine. DOTAP Liposomal Transfection Reagent is a liposome formulation of the cationic lipid DOTAP. In addition, a proteomic analysis revealed that these responses are mirrored by changes in the plant proteome, such as up-regulation of proteins related to defense responses, including proteins involved in photorespiration, cysteine and oxylipin synthesis, and oxidative stress response; and down-regulation of enzymes related to photosynthesis. We are currently working diligently to update our website pricing information for the New Year. by 2,3-bis[[(Z)-octadec-9-enoyl]oxy]propyl-trimethylazanium;methyl sulfate. Register a free CiteAb account today to view them. provision, helping them make more informed business decisions and drive increased sales. by See how others have used DOTAP Transfection Reagent(CAS144189-73-1). alScientific reports, 6, undefined (2016-3-17), Adobe Acrobat Reader is required to reliably view, print and comment on PDF documents. Multifunctional Immunoadjuvants for Use in Minimalist Nucleic Acid Vaccines.
Epub 2019 Mar 12. on 15 March 2022 DOTAP MS (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate) is a proven reagent for the highly efficient liposomal transfection of DNA, RNA, oligonucleotides, and some other negatively charged large biomolecules. Many experiments with DOTAP, as one of the longest-standing known "first-generation" reagents, are described in the scientific literature available, both in vitro & in vivo. and quantity. NPP1, a Phytophthora-associated trigger of plant defense in parsley and Arabidopsis. 2017 Dec;246(6):1109-1124. doi: 10.1007/s00425-017-2755-z. Four liposome formulations with different 1,2-dioleoyl-3-trimethylammoniumpropane (DOTAP) to dioleoylphosphatidylethanolamine (DOPE) weight ratios were investigated, that is, weight ratios 1:0 (T1P0), 3:1 (T3P1), 1:1 (T1P1), and 1:3 (T1P3). PICT2013-0625/Fondo para la Investigacin Cientfica y Tecnolgica, Plant Physiol. Santa Cruz Biotechnology, and the Santa Cruz Biotechnology, Inc. logo, Santa Cruz Animal Health, San Juan Ranch, Supplement of Champions, the San Juan Ranch logo, UltraCruz, ChemCruz, ImmunoCruz, ExactaCruz, and EZ Touch are registered trademarks of Santa Cruz Biotechnology, Inc. All trademarks are the property of their respective owners. on 23 March 2021 In The unsatisfactory and unreliable results of the calcium phosphate transfection method led to the development of transfection reagents of the 1. Furthermore, DOTAP was able to prime the defense response for later pathogenic challenges as in the case of the virulent bacterial pathogen Pseudomonas syringae pv. Response of human macrophages to gamma radiation is mediated via expression of endogenous retroviruses. A key feature of plant immunity is the ability to sense potentially dangerous signals, as it is the case for microbe-associated, pathogen-associated or damage-associated molecular patterns and by doing so, trigger an active defense response to cope with the perturbing stimulus. DODMA is highly efficient at encapsulating nucleic acid to form lipoplexes during synthesis by reducing the physiological pH, which readily passes through the cell membrane. The .gov means its official. Not for use in diagnostic procedures. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded. Click the "Request Quote" button at the bottom of the Shopping Cart page. Disclaimer, National Library of Medicine By signing up, you indicate that you have read and agree to the terms in our privacy policy. Zahid A, Jaber R, Laggoun F, Lehner A, Remy-Jouet I, Pamlard O, Beaupierre S, Leprince J, Follet-Gueye ML, Vicr-Gibouin M, Latour X, Richard V, Guillou C, Lerouge P, Driouich A, Mollet JC. 2002 Mar;128(3):1046-56 Use approximately 5-10 g DOTAP per g DNA and approximately 10-20 g DOTAP per mL cell culture medium. It can also be successfully used for in vivo applications. PLoS Pathogens You can add items to your cart right from here. citation Product Type: Chemical. 
We support reagent suppliers with our In addition, serum inhibition is reported for many of these transfection reagents with the exception of DOTAP. Therefore, DOTAP may be considered a good candidate as an elicitor for the study of plant immunity. on 25 October 2021 You have the right to unsubscribe at any time by clicking the 'unsubscribe' link in any News & Announcement emails you receive. dotap reagent transfection 2010 Jan;152(1):267-80. doi: 10.1104/pp.109.148049. This method of DNA transfer is very gentle, avoiding the cytotoxic effects commonly encountered with lipofection or other transfection methods. 2021 May 1;13(5):644. doi: 10.3390/pharmaceutics13050644. 2005 Feb;41(3):451-63 Call us Toll Free at 1-800-457-3801. DOTAP Transfection Reagent is a liposomal formulation of the cationic lipid DOTAP. DOTAP 1 mlTransfection reagent for mammalian cells, DOTAP 2 x 1 mlTransfection reagent for mammalian cells, DOTAP 5 x 1 mlTransfection reagent for mammalian cells, By continuing to browse this website, you agree to the use of cookies Read more Clipboard, Search History, and several other advanced features are temporarily unavailable. As a rule the proportion of DNA to lipids must be optimized carefully to ensure satisfactory results. VAT #179706954. Spotted a mistake? JavaScript seems to be disabled in your browser. Disruption of poly(ADP-ribosyl)ation mechanisms alters responses of Arabidopsis to biotic stress. 2004;55:373-99 on 3 November 2020 DOTAP Transfection Reagent is also suitable for other negatively charged molecules, including oligonucleotidees and glutamic acid. on 1 December 2020 2020 Jan 8;20(1):16. doi: 10.1186/s12870-019-2232-x. Size and zeta potential of liposomes were changed by preparation concentration. The proven first-generation transfection reagent DOTAP (1,2-Di-(9Z-octadecenoyl)-3-trimethylammonium propane methylsulfate) in sterile water (concentration 1 mg/ml) is supplied by Biontex as a ready-to-use liposome formulation. on 1 October 2020 Our The shape of lipoplexes was changed from lamellar to inverted hexagonal structure according to the increased ratio of DOPE, but there was no definite advantage of specific structure in transfection efficiency throughout all used cell lines. We use cookies on this site to enhance your user experience. Cationic lipid that can be used as an effective transfection reagent in a variety of cell lines/cell types grown in suspension and adherent cultures. Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Our findings show that the transfection efficiency of DOTAP liposomes is mainly influenced by lipid composition and cell type, and not by size or zeta potential. Tweedell, R. E., Malireddi, R. K. S., et al.. A collection of useful calculators with more to come! and In understand the privacy policy. A further feature of these reagents is a generally conventional transfection sequence providing for cell seeding and growth prior to the actual transfection and for change of medium after transfection. Although DOTAP does not offer all the features of modern transfection reagents, in many cases it delivers reliable results. MeSH Commentary on Grandellis et al. This site needs JavaScript to work properly. Unlike other liposomal reagents, it produces highly efficient transfections both in the presence and absence of serum. Not Intended for Diagnostic or Therapeutic Use. alNature, 474(7349), undefined (2011-5-10)3. Click on the entry to view the PubMed entry To keep up to date with the latest developments to our search engine, news from our life science market In Generation based on cationic lipids or liposomes. Joshi, H., Almgren-Bell, A., et al.. DOTMA Chloride (N-[1-(2,3-Dioleyloxy)propyl]-N,N,N-trimethylammonium (chloride)) is a proven reagent for the highly efficient liposomal transfection of DNA, RNA, oligonucleotides, and other negatively charged large biomolecules. official website and that any information you provide is encrypted Molecular Weight: 774.19
Influence of transfection time on CAT expression. Arabidopsis thaliana; Cationic lipid; DOTAP; Plant defense responses. They do not feature buffering properties and therefore do not offer the option of endosome buffering, which explains their lower efficiency compared to modern reagents (e.g. sharing sensitive information, make sure youre on a federal For the best experience on our site, be sure to turn on Javascript in your browser. Transfection efficiencies were determined mainly by lipid compositions. Genetic buffering of cyclic AMP in Arabidopsis thaliana compromises the plant immune response triggered by an avirulent strain of Pseudomonas syringae pv. All Rights Reserved. Here, we evaluated the effect of DOTAP in plant basal innate immunity. on 1 December 2020 Salmon, J. M., Todorovski, I., et al.. L-plastin enhances NLRP3 inflammasome assembly and bleomycin-induced lung fibrosis. and transmitted securely. -, Plant J. Add multiple items to your shopping cart by entering the Krackeler Cat.No. You must have JavaScript enabled in your browser to utilize the functionality of this website. METAFECTENE). 
DOTAP Chloride (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride) is a widely used cationic liposome-forming compound for in vitro and in vivo transfection of DNA, RNA and other negatively charged molecules.